LifeB | Laboratory of interaction and function of essential biomolecules

What we use in Laboratory

High-end fluorescence microscopy

We observe dynamics and protein binding directly in live cell using Fluorescence correlation Spectroscopy (FCS) and Fluorescence Recovery After Photobleaching (FRAP).

Specimen requirements

  • One or two molecules fluorescently labeled by transient expression of fluorescent proteins in a stable cell line
  • Live cells grown on a dish

Fluorescence anisotropy with automated mixing and titration

We measure precisely binding affinity of fluorescently labelled proteins and nucleic acids by automatic detection of slower rotational movement of biomolecules after protein-protein* and protein-DNA* formation using fluorescence anisotropy.

Sample requirements

  • One molecule is fluorescently labeled
  • Sample consumption is in the range of 10 -100μg

Isothermal titration calorimetry

We describe heat evolved or consumed during biomolecule biding. From one measurement of isothermal titration calorimetry, we can obtain full thermodynamic profile of reaction.

Sample requirements

  • No labeling of molecules needed
  • Sample consumption 0.1 - 1 mg

Fully automated western blotting

We use fully automated western procedures that make long and boring blotting to be real fast and fun.

Sample requirements

  • Disposable cassettes for membrane washing
  • 10 mL of antibody solutions